CRISPR/Cas9 Genetic Engineering – How Gilson Manual and Automated Pipetting Tools Facilitate the Process
CRISPR/Cas9 is undoubtedly one of the most revolutionary tools in modern molecular biology with wide reaching applications for basic science, industry and healthcare. Within only a few years after its initial discovery as a programmable gene-editing technique, CRISPR/Cas9 has fast become the method of choice for genome engineering in many laboratories all over the world.
Gilson provides scientists with a range of manual and automated high precision liquid handling tools to enable CRISPR/ Cas9 gene editing research. By choosing the right tool for the right job, convenience, productivity, accuracy and precision can all be maximised to ensure reproducible and verifiable results throughout the CRISPR workflow.
As with many techniques in modern molecular biology, CRISPR/ Cas9 workflows are dominated by pipetting, liquid handling and the complexing and delivery of key reagents. Based on Gilson’s longstanding expertise in the development of liquid handling and laboratory automation, our products offer the high level of accuracy and precision required for all levels of a CRISPR/Cas9 experimental workflow.
The Gilson PIPETMAN® L Multichannel or new electronic Bluetooth®-enabled PIPETMAN® M Connected Multichannel ranges make setting up multiple 96-well plates for clonal cell culture straightforward and fast, giving you the maximum probability of detecting your modification of interest.
PLATEMASTER® is the ideal solution for performing 96-well or 384-well plate-toplate transfers or for transferring buffers or media from a reservoir. With zero programming, minimal set-up is required, and its small footprint makes it ideal to use in a laminar flow hood or cabinet.
For the sample preparation and qPCR validation of clones, the Gilson PIPETMAX® automated liquid handler removes the risk of manual error. With its volume range from 1 µL to 1 mL, and the ability to use 1 to 8 pipetting tips at any one time, PIPETMAX® is the most versatile liquid handler for its size. PIPETMAX® can eliminate user to user error, as well as human error in sample transfers, while completing experiments unattended, leaving scientists more time to focus on more important tasks. The ability to use 96-and 384-well plates, allows for increased throughput with sample tracking. PIPETMAX® uses the same tips as the manual Gilson pipettes so you can be assured of the same excellent performance.
In addition to the PIPETMAN® L and PIPETMAN® M manual and electronic pipettes, PIPETMAX® offers industryleading accuracy and precision and is the perfect accompaniment for automating your qPCR validation experiments. The Normalization Assistant, a software wizard, allows you to import your DNA concentrations: PIPETMAX® automatically calculates the volumes required to be transferred to create your normalized plate. In addition, the qPCR Assistant, also a software wizard, helps you to set up your qPCR experiment exactly how you want it and keep track of numerous samples with ease. Its flexibility allows you to create and store multiple experiments and use our preferred labware, while creating an output file that can be imported directly to your thermocycler.
OPTIMISE YOUR WORKFLOW
-
SMART, MOTORISED PIPETTES
Get accuracy and precision with the new Bluetooth®-enabled PIPETMAN® M Connected -
MICROPLATE PIPETTING
Fill 96- or 384-well plates quickly and accurately with PLATEMASTER® -
FULLY AUTOMATED PIPETTING
Maximise reproducibility in your biological samplepreparation with PIPETMAX®
For further information, download the full Tech-Note on CRISPRCas9 Genetic Engineering at go.gilson.com/CRISPR-TN or visit www.gilson.com
Download our Pipetting Workflow Guide here.
